王贤纯

来源:湖南师范大学生命科学学院   2013-7-10 17:27:28   浏览:2490

 
      名:王贤纯
      科:生物化学与分子生物学
研究方向:蛋白质化学与蛋白质组学
电话/传真: 0731-88872556
电子邮件: wang_xianchun@263.net
通讯地址:湖南省长沙市河西,湖南师范大学生命科学学院
邮政编码: 410081
 
个人简介
1959年4月出生,1977年参加工作,1982年获学士学位,1986年获硕士学位,1998年获博士学位。现为湖南师范大学二级教授,博、硕士生导师;湖南省生物化学与分子生物学会理事,中国生物化学与分子生物学会第三届蛋白质组学专业委员会委员;蛋白质化学与发育生物学教育部重点实验室副主任、第三届学术委员会委员;湖南省生物化学与分子生物学重点实验室、教育部蛋白质化学与发育生物学重点实验室和省部共建国家重点实验室培育基地主要学术带头人之一;湖南师范大学第六届“十佳师德标兵”。长期致力于生物化学及相关学科的教学与教研、科学研究、学科建设与人才培养及实验室建设工作。主讲生物化学和蛋白质技术等课程,曾获生命科学学院教师教学竞赛一等奖和湖南师范大学第四届青年教师课堂教学艺术竞赛一等奖。一直担任生物化学与分子生物学系主任,具体负责该系的本科教学及相关工作,带领该系进入湖南师范大学“三育人”先进单位行列。主要从事蛋白质化学、蛋白质组学和天然蛋白质多肽毒素等方面的研究。多次应邀在国际国内学术会议上做学术报告。曾先后前往美、英、韩国和越南参加短期学习和学术交流。主持国家863课题和科技部重大基础研究前期研究专项课题各1项,国家自然科学基金项目课题4项,教育部高等学校博士学科点专项科研基金课题2项,湖南省自然科学基金重点课题和一般课题等省级课题4项。以通讯作者或第一作者在J Proteome Res, Mol Brain, PloS One, Toxins, ElectrophoresisInt J Biol Macromol,生物化学与生物物理学报和中国生物化学与分子生物学报等国内外重要学术刊物上发表科研和教研论文90余篇,获授权国家发明专利2项,受理1项, 通过省级专家鉴定成果1项。指导培养博士、硕士研究生30余名。参编著作4部。作为主要完成人之一获2004年和2007年湖南省科技进步一等奖。
 
代表性论著 (通讯作者或第一作者)
1. Xiaozhen Peng, Zhipan Dai, Qian Lei, Long Liang, Shuai Yan and Xianchun Wang. Cytotoxic and apoptotic activities of black widow spiderling extract against HeLa cells. Exp Ther Med. 2017, (In proof)
2. Tinayao Guo, Zhigui Duan, Jia Chen, Chunliang Xie, Ying Wang, Ping Chen, and Xianchun Wang. Pull-down combined with proteomic strategy reveals functional diversity of synaptotagmin I. PeerJ, 2017, 5:e2973.
3. Xia Tang, Chunliang Xie, Ying Wang, Xianchun Wang. Localization of Rab3A-binding site on C2A domain of synaptotagmin I to reveal its regulatory mechanism. Int J Biol Macromol. 2017, 96:736-742.
4. Dehong Xu and Xianchun Wang. Transcriptome Analysis to Understand the Toxicity of Latrodectus tredecimguttatus Eggs. Toxins, 2016, 8(12):378-400.
5. Xia Tang, Jia Chen, Ying Wang, Xianchun Wang. Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis. SpringerPlus, 2016, 5(1):1705-1711.
6. Shuai Yan and Xianchun Wang. Recent Advances in Research on Widow Spider Venoms and Toxins.Toxins, 2015, 7, 5055–5067.
7. Hai Yu, Hui Liu, Yi-Zhong Yan, Zhi-Pan Dai, Shuai Yan, Jiang-Lin Li, Zhi-Gui Duan, Zhen  
Liu, Xian-Chun Wang. Development and application of wide-range gradient gel electrophoresis to proteome analysis. Anal Methods. 2015, 7:8109-8115.
8. Si-Ling FU, Jiang-Lin LI, Jia CHEN, Qiu-Ting WANG, Jian-Jun LI, Xian-Chun WANG1. Extraction and identification of membrane proteins from black widow spider eggs. Zool Res. 2015,36(4): 248-254.
9. Qian Lei, Hai Yu, Xiaozhen Peng, Shuai Yan, Jirong Wang, Yizhong Yan, 
 Xianchun Wang. Isolation and Preliminary Characterization of Proteinaceous Toxins with Insecticidal and Antibacterial Activities from Black Widow Spider (L. tredecimguttatus) Eggs. Toxins, 2015,7:886-899.
10. Chen J, Dai Z, Lei Q, Yan S, Wang Y, Wang X.Effects of huwentoxin-I on catecholamines in    
 cultured PC12 cells. Toxicol Mech Methods. 2015, 25(4):241-8.
11. Yizhong Yan, Jianjun Li, Yiya Zhang, Xiaozhen Peng, Tianyao Guo, Jirong Wang, Weijun
   Hu, Zhigui Duan and Xianchun Wang. Physiological and biochemical characterization of egg
   extract of black widow spiders to uncover molecular basis of egg toxicity. Biol Res. 2014,
   17-27.
12. Hai Yu, Hui Liu, Yizhong Yan, Zhigui Duan and Xianchun Wang . Detection and identification of huwentoxin-IV interacting proteins with biotin-avidin chemistry combined with mass spectrometry. J Venom Anim Toxins Incl Trop Dis. 2014, 20:18-26.
13. Peng X, Zhang Y, Liu J, Yu H, Chen J, Lei Q, Wang X, Liang S. Physiological and Biochemical Analysis to Reveal the Molecular Basis for Black Widow Spiderling Toxicity.J Biochem Mol Toxicol. 201428(5):198-205.
14. Xie C, Li J, Guo T, Yan Y, Tang C, Wang Y, Chen P, Wang X, Liang S. Rab3A is a new interacting partner of synaptotagmin I and may modulate synaptic membrane fusion through a competitive mechanism.Biochem Biophys Res Commun. 2014,444(4): 491-495.
15. Li J, Yan Y, Yu H, Peng X, Zhang Y, Hu W, Duan Z, Wang X, Liang S. Isolation and identification of a sodium channel-inhibiting protein from eggs of black widow spiders. Int J Biol Macromol. 2014, 65:115-120.
16. Xie C, Su H, Guo T, Yan Y, Peng X, Cao R, Wang Y, Chen P, Wang X, Liang S. Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus. Mol Brain. 2014, 7(1):4-16.
17. Jianjun Li, Hai Yu, Zhigui Duan, Xianchun Wang. A sample preparation method for
micro-scale membrane proteome analysis. Sam Prep. 2013, 28-36.
18. Yi-Zhong Yan, Hui Liu, Rong Lv, Sha Huang, Ji-Rong Wang, Tian-Yao Guo, Qiong Zhou and Xian-Chun Wang. Biotin labeling and receptor analysis of huwentoxin-I. Anal Methods, 2013, 5, 3888-3894.
19. Jianjun Li, Yizhong Yan, Jirong Wang, Tianyao Guo, Weijun Hu, Zhigui Duan, Xianchun Wang, and Songping Liang. Purification and Partial Characterization of a Novel Neurotoxic Protein from Eggs of BlackWidow Spiders (Latrodectus tredecimguttatus). J Biochem Mol Toxicol. 2013, 27(7): 337-342.
20. Yong Lin, Linju Huo, Zhonghua Liu, Jianglin Li, Yi Liu, Quanze He, Xianchun Wang, Songping Liang.Sodium Laurate, a Novel Protease- and Mass Spectrometry-Compatible Detergent for MassSpectrometry-Based Membrane Proteomics. PLoS One 2013, 8(3) : e59779.
21. Lin Y, Liu H, Liu Z, Liu Y, He Q, Chen P, Wang X, Liang S. Development and evaluation of an entirely solution-based combinative sample preparation method for membrane proteomics.Anal Biochem. 2013, 432 (1): 41-48.
22. Li J, Liu H, Duan Z, Cao R, Wang X, Liang S. Protein Compositional Analysis of the Eggs of Black Widow Spider (L. tredecimguttatus): Implications for the Understanding of Egg Toxicity. J Biochem Mol Toxicol. 2012, 26(12):510-515.
23. Xianchun Wang, Songping Liang. Sample preparation for the analysis of membrane proteomes by mass spectrometry. Protein & Cell. 2012, 3(9):661-668.
24. Yong Lin, Hui Liu, Zhonghua Liu,Xianchun Wang, Songping Liang. Shotgun analysis of membrane proteomes using a novel combinative strategy of solution-based sample preparation coupled with liquid chromatography-tandem mass spectrometry. J Chromatogr B, 2012, 901:18-24.
25. Xianchun Wang and Songping Liang. Gel-absorption-based sample preparation method for shotgun analysis of membrane proteome. Methods Mol Biol. 2012, 869:385-392.
26. Jian Zhou, Deng Bi, Yong Lin, Ping Chen, Xianchun Wang, Songping Liang. Shotgun proteomics and network analysis of ubiquitin-related proteins from human breast carcinoma epithelial cells. Mol Cell Biochem. 2012, 359: 375-384.
28. Jian Zhou, Jianlin Li, jianjun Li, Ping Chen, Xianchun Wang X, Songping Liang S. Dried polyacrylamide gel absorption: A method for efficient elimination of the interferences from SDS-solubilized protein samples in mass spectrometry-based proteome analysis. Electrophoresis, 2010313816-3822.
29. Yong Lin, Yi Liu, Jianjun Li, Yan Zhao, Quanze He, Wenjun Han, Ping Chen, Xianchun Wang , Songping Liang. Evaluation and optimization of removal of an acid-insoluble surfactant for shotgun analysis of membrane proteome. Electrophoresis, 2010312705-2713.
30. Zhou J, Xiong J, Li J, Huang S, Zhang H, He Q, Lin Y, Chen P, Wang X, Liang S. Gel absorption-based sample preparation for the analysis of membrane proteome by mass spectrometry. Anal Biochem. 2010404(2):204-10.
31. Jian Zhou, Sha Huang, Deng Bi, Hai Zhang, Jianglin Li, Yong Lin, Ping Chen, Xianchun Wang, Songping Liang.Analysis of integral membrane proteins by heat gel-embedment combined with improved in-gel digestions. Electrophoresis, 2009,30 (23):4109-4117.
32. Xiong Xia, Shen Jian-Ying, Li Jian-Jun, Zhang Hai, Wang Xian-Chun, Liang Song-Ping. Aqueous polymer two-phase partition for the proteomic analysis of plasma membrane from rat dorsal root ganglion neurons. Prog Biochem Biophys, 2009, 36(11):1458-1468.   
33. Xia Xiong, Sha Huang, Hai Zhang, Jian-jun Li, Jian-Ying Shen, Jixian Xiong, Yong Lin, Liping Jiang, Xian-Chun Wang, Song-Ping Liang. Enrichment and proteomic analysis of plasma membrane from rat dorsal root ganglion neurons. Proteome Sci. 2009,7:41-50.  
34. Yong Lin, Yan Li, Yi Liu, Wenjun Han, Quanze He, Jianglin Li, Ping Chen, Xianchun Wang , Songping Liang . Improvement of gel-separated protein identification by DMF-assisted digestion and peptide recovery after electroblotting. Electrophoresis, 2009,30(20):3626-3635.   
35. Zhigui Duan, Xiaojun Yan, Rui Cao, Zhen Liu, Xianchun Wang and Songping Liang.Proteomic analysis of Latrodectus tredecimguttatus venom for uncovering potential latrodectism-related proteins. J Biochem Mol Toxicol. 2008,22(5):328-336.    
36. Yong Lin, Jian Zhou, Deng Bi, Ping Chen, Xianchun Wang, Songping Liang . Sodium deoxycholate- assisted tryptic digestion and identification of proteolytically resistant proteins. Anal Biochem. 2008, 377(2): 259266.    
37Jian Zhou, Yong Lin, Xingcan Deng, Jianying Shen, Quanyuan He, Ping Chen,Xianchun Wang, Songping Liang. Development and application of a two-phase, on-membrane digestion method in the analysis of membrane proteome. J Proteome Res. 2008, 717781783.  
38X.C. Wang, Z.G Duan, J.Yang, X.J.Yan, H. Zhou, X.Z.He, and S.P.Liang. Physiological and biochemical analysis of L. tredecimguttatus venom collected by electrical stimulation. J Physiol Biochem. 2007, 63(3):221-230    
39. Jian Zhou,Tieyang Zhou, Rui Cao, Zhen Liu, Jianying Shen, Ping Chen, Xianchun Wang, and Songping Liang.Evaluation of the application of sodium deoxycolate to proteomic analysis of rat hippocaampal plasma membrane. J Proteome Res.2006, 5, 2547-2553。   
40. Duan ZG,Yan XJ,He XZ,Zhou H,Chen P,Cao R,Xiong JX,Hu WJ,Wang XC,Liang SP.Extraction and protein component analysis of venom from the dissected venom glands of Latrodectus tredecimguttatus. Comp Biochem Physiol. Part B. 2006,1453-4):350-357  
41. Wang lin, Zhou Tieyang, Tang Xing, Wang Xianchun,Liang Songping.Purification and characterization of a novelα-amylase inhibitor from wild Amaranth (Amaranthus paniculatus) weeds. Chin J of Biochem Mol Biol, 2004,20(4):434-439  
42. WANG Xian-Chun, CHEN Ping, LIANG Song-Ping. Liquid chromatography-tandem mass spectrometry analysis of a synthetic nonapeptide and its main by-products. Chin J Biochem Mol Biol, 2003195):576-581.
43. 王贤纯,梁宋平。间斑寇蛛毒素的蛋白质化学与蛋白质组学研究新进展。生物工程学报, 2014,30(7):1036-1043。
44. 王贤纯 陈 平王迎 王美迟 曾雄智 梁宋平。生物化学双语教学的课堂实践与探索。教学研究。2013,36(5):57-60。
45. 王贤纯,陈 平,王 迎,王美迟,曾雄智,段志贵,刘 珍,梁宋平。生物化学双语教材建设的探讨。高师理科学刊。2012,32(1):101-103。
46. 段志贵,杨静,晏晓军,曾雄智,王贤纯,梁宋平。间斑寇蛛粗毒液的生理生化分析及两种采毒方法的比较。动物学研究,2009,30(4):381-388。
47. 林勇,周健,毕登,陈平,王贤纯,梁宋平。脱氧胆酸钠(SDC)促进的蛋白质的酶解与鉴定。中国科技论文在线精品论文。2009,2(7):669-673。
48. 王贤纯, 陈平, 王迎,曾雄智, 王美迟,梁宋平。关于进一步推进高校本科双语教学的几点思考。高等教育研究学报,2008,31(2):36-37。
49. 王贤纯, 陈平, 曾雄智, 王美迟。 高校双语教学的实施策略。北京大学学报(哲学社会科学版),2007,S2:68-69。
50. 杨静,段志贵,周红,晏晓军,何小珍,王贤纯,梁宋平。间斑寇蛛粗毒的生物学特性。动物
   学报,2007,53(4):682-688。
51. 段志贵,晏晓军,邓星灿,王贤纯。间斑寇蛛幼蛛过冬及其早期饲养管理。生态学杂志
   2007,26(3):363-367
52.晏晓军,段志贵,周远峰,王贤纯,梁宋平。间斑寇蛛(L tredecimguttatus)粗毒抗血清
   制备及其活性分析。蛛形学报,2006,22(2)80-84。
53. 周铁杨,周健,曹锐,沈建英, 陈平, 王贤纯。大鼠海马细胞质膜的分离及其蛋白质组学分析。中国生物化学与分子生物学报,2006,22(9):733-743。
54. 周铁杨,周健,雄继先,王贤纯。基于等电聚焦-反相HPLC的虎纹捕鸟蛛毒素组学的
   初步研究。中国生物化学与分子生物学报,2006,22(10):823-831。
55. 范春明,王贤纯,梁宋平。两种蜘蛛多肽毒素的质谱法序列分析。质谱学报。2005,26(2):65-70。
56. 徐峡,熊霞,李冬玲,肖玉成,王贤纯,梁宋平。海南捕鸟蛛毒素-IV突变体的固相合成和生理活性分析。生物工程学报,2005,21(1):92-96。
57. 曹锐,张丽军,聂松,王贤纯,梁宋平。多维色谱-串联质谱联用技术分离和鉴定小鼠肝脏质膜蛋白质。中国生物化学与分子生物学报。2005,21(1):134-142。
58. 周红,何小珍,段志贵,晏晓军,胡绍海,王贤纯,梁宋平。间斑寇蛛(L tredecimguttatus) 室内饲养及活体采毒方法的初步探讨。蛛形学报,2005,14(1):58-63。
59. 王贤纯,梁宋平。虎纹捕鸟蛛毒素-I Lys13突变对生物学活性的影响。自然科学进展
   2004,14(2):227-231
60. 王贤纯,梁宋平。反相高效液相色谱/电喷雾质谱法分析化学合成七肽粗产物。分析化学。2004,32(9):1219-1222。
61. 王贤纯,范春明,唐新科,陈平,梁宋平。牛血清白蛋白胰蛋白酶酶解产物的色谱-质谱联用分析及其三种数据库搜寻鉴定方法的比较。中国生物化学与分子生物学报。2004,20(3):393-398。
62. 王贤纯,梁宋平。化学合成ω-芋螺毒素MVIIA的复性与质谱分析。动物学研究。2003,24(2):121-126。
63.王贤纯。蜈蚣的药用研究进展。动物学杂志,2002,37(3):88-91。
64. 王贤纯,梁宋平。电喷雾串联质谱图的叠合与多肽序列分析。生物化学与生物物理学报。2001,33(6):665-670。
65. 王贤纯,梁宋平。化学合成虎纹捕鸟蛛毒素-I的色谱行为及分离纯化。色谱。2001,19(2):101-104。
66. 王贤纯,梁宋平,罗泽民。虎纹捕鸟蛛毒素-I单残基突变体R20A-HWTX-I的化学合成与性质分析。生物工程学报。2000,16(4):490-494。
67. 王贤纯,梁宋平,罗泽民。构建同时携带HWTX-I和AAI活性位点的多肽嵌合体。生物化学与生物物理学报,2000,32(3):274-280。
68. 王贤纯,梁宋平,罗泽民。虎纹捕鸟蛛毒素-I突变体A1Y-HWTX-I的固相合成和生物学活性测定。中国生物化学与分子生物学报,2000,16(3):357-362。
69. 王贤纯,梁宋平。不同溶剂对合成多肽的洗脱效果比较。化学通报(网络版)。1999,c00083。
70. 王贤纯,梁宋平。豆科植物籽实的昆虫消化道α-淀粉酶抑制剂的研究(II)。生物技术。1995,5(2):29-31。
71. 王贤纯,梁宋平。豆科植物籽实的昆虫消化道α-淀粉酶抑制剂的研究(I)。生物技术。1994,4(5):18-21。
72. 王贤纯,谢锦云,单茶秀,戚志仁。姜黄的综合利用—水蒸汽蒸馏法提取姜黄油。食品科学。1993,8:49-51。
73. 王贤纯,谢锦云,单茶秀,戚志仁。姜黄的综合利用—乙醇循环法提取姜黄素。食品科学。1993,9:45-47。
74. 王贤纯。姜黄色素的稳定性。食品与发酵工业。1994,1:63-66。

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